From the Wellcome Trust Sanger Institute, these resources will help students to understand the processes involved in the sequencing of DNA and the Human Genome Project. Animations illustrate: * The Human Genome Project (HGP) * DNA sequencing * Producing DNA libraries * Subcloning of DNA The information is quite detailed and suitable for students following a post-16 course in biology. To run the animations, they must first be downloaded and saved on your computer. They will then run as Flash animations.

Please note: From 2021, Adobe has discontinued support for Flash player and as a result some interactive files may no longer be playable. As an alternative method to accessing these files a group of volunteers passionate about the preservation of internet history have created project Ruffle (https://ruffle.rs/). Ruffle is an entirely open source project that you can download and run many interactive Flash resources. For further information regarding STEM Learning’s policy for website content, please visit our terms and conditions page.

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DNA Libraries: Bacterial Artificial Chromosome (BAC)

Produced by the Wellcome Trust Sanger Institute, this resource contains an overview of the techniques involved in making a BAC (Bacterial Artificial Chromosome) library. It consists of an animation that covers the key stages in the process:

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Subcloning

This animation shows the major stages of subcloning. This is the process of breaking up fragments of DNA from BAC clones into smaller more manageable pieces for DNA sequencing. This process was very important during the Human Genome Project. Subcloning involves several standard molecular biology techniques...

DNA Sequencing

From the Wellcome Trust Sanger Institute, this animation shows a detailed overview of the processes involved in the dideoxy or Sanger method for sequencing DNA. This is the method used to sequence the human genome. The animation demonstrates the principles and techniques involved in the Sanger sequencing method,...

How the Human Genome Was Sequenced

From the Wellcome Trust Sanger Institute, this resource introduces the Human Genome Project (HGP).

A short animation gives:

* an introduction to DNA and genomes;

* an overview of which countries were involved in the international Human Genome Project;

* a description of the processes,...