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Catechol Oxidase Activity in Fruits and Vegetables

Produced by Science & Plants for Schools (SAPS), this investigation looks at the activity of the enzyme catechol oxidase (also called polyphenoloxidase). Found in a wide variety of plants, it is responsible for the darkening observed when many fruits or vegetables are cut or bruised.

Details of the materials needed and a suitable method to assay the enzyme activity are included.

Possible investigations include:
• measuring the catechol oxidase activity of a fruit at different stages of ripeness
• measuring the catechol oxidase activity of different varieties of the same fruit
• measuring the catechol oxidase activity of different fruits.

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