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These resources have been reviewed and selected by STEM Learning’s team of education specialists for factual accuracy and relevance to teaching STEM subjects in UK schools.

Chromatography

Chromatography is one of the titles in the series of ASE Lab Books that were published in the early 1970s for the Association for Science Education by John Murray. Each title covered one or two topics and brought together the best of the teaching notes and experimental ideas from members of the association that had appeared in the ASE journal, School Science Review.

The sections of this resource and the topics are as follows.

Paper chromatography
* Paper chromatography for junior schools
* Test-tube chromatography
* Paper chromatography of felt-tip pens
* Simple experiments in chromatography
* Separation of laboratory indicator dyes
* Some uses of chromatography in botany
* Enzyme action illustrated by paper chromatography
* Studies relating to Capsicum annuum (red and green peppers) ,

* Paper chromatography of monosaccharides
* Paper chromatography of lipids
* Paper chromatography of protein hydrolysates
* Food colouring in Smarties
* Confirmation of sodium, potassium and magnesiumby paper chromatography
* Confirmation of the presence of chloride, bromide and iodide ions in qualitative analysis by paper
chromatography
* Identification of a Group 2A precipitate
* Identification of a Group 4 precipitate
* Tables useful in the separation and identification of inorganic ions by paper chromatography ,

Thin-layer chromatography
* Preparation of chromatostrips and detection of components
* Preservation of specimens
* Spreading.and preparation of large plates
* Solvents that can be used for elution
* Solvent effect in T.L.C.
* The separation of ink pigments
* Separation of cis- and trans-isomers of azobenzene
* Separation of the indicators in Universal Indicator
* Monitoring the esterification of benzoic acid
* The identification of amino acids in a mixture
* Separation of inorganic ions
* Separation of phenols
* The effect of acid on a condensation reaction of propanone (acetone)
* Chromatographic investigation of the products of nitration of phenol
* The nitration of phenol
* T.L.C. of chlorophyll pigments
* T.L.C. of plant acids
* T.L.C. of amino acids
* T.L.C. of sugars
* Isolation of organochlorine pesticide residues from carrots

Column chromatography
* Preparation of the column
* Experiments for class use
* Test-tube chromatography and ion-exchange
* Preparation of 2,4-dinitrophenylhydrazones
* 'Dry column' chromatography
* Chromatographic separation of yellow plastid pigments
* Molecular sieve chromatography
* Gel permeation chromatography

Gas chromatography
* Simple experiments using the principles of gas chromatography
* Gas chromatography as a class experiment
* An apparatus for gas chromatography
* Experimental gas chromatography
* A gas chromatography injection head to prevent leakage
* Experiments with the gas chromatography apparatus

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