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Science & Plants for Schools (SAPS)
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Investigating enzymes: phosphatase enzymes in plants
One of a series of resources from Science & Plants for Schools (SAPS) investigating key topics in plant biology. A standard protocol which is used to extract phosphatase enzymes from plants, including a method to calculate the rate of reaction. Phosphatases are enzymes widespread in plants easily extracted from germinating seeds. The pink substrate phenolphthalein phosphate (PPP) can be used to estimate the amount of enzymatic activity, and investigations can be carried out under differing pH and temperatures. Students have the opportunity to use colorimeters in this practical.
The resource is suitable for use for A level, Scottish Highers and IB.
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Phosphatase enzymes (worksheet) 101.5 KB
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